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. 2021 Oct 6;9:709574. doi: 10.3389/fcell.2021.709574

FIGURE 2.

FIGURE 2

Effects of chaetocin, TSA, and the combination on the developmental competence of porcine SCNT embryos. (A) Representative immunofluorescent images of CDX2/DAPI-stained blastocysts. Green and blue fluorescence indicate CDX2 and nuclei, respectively. Bar = 50 μm. Quantification of (B) ICM and TE cell numbers and (C) ICM/TE ratios (n = 20 per group). (D) qRT-PCR data on embryonic development-related gene expression in blastocysts (n = 3 per group). (E) Representative TUNEL assay images of blastocysts. Green and blue fluorescence indicate the TUNEL label (white arrow) and nuclei, respectively. Bar = 50 μm. Quantification of (F) the apoptotic cell number and (G) the apoptosis rate (n = 20 per group). (H) qRT-PCR data on apoptosis-related gene expression in blastocysts (n = 3 per group). The data are from three independent experiments and are means ± SEM. Values with different superscripts differ significantly (P < 0.05). Con, control; CH, chaetocin; T, TSA; ICM; inner cell mass; TE, trophectoderm cell.