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. 2021 Oct 6;9:709574. doi: 10.3389/fcell.2021.709574

FIGURE 4.

FIGURE 4

Effects of chaetocin, TSA, and the combination on H3K9ac levels in porcine SCNT embryos. (A) Representative immunofluorescent images of H3K9ac at the pronuclear stage. Green and blue fluorescence indicate H3K9ac and nuclei, respectively. Bar = 50 μm. (B) Quantification of fluorescence intensity at the pronuclear stage (n = 20 per group). (C) Representative immunofluorescent images of H3K9ac at the two-cell stage. Green and blue fluorescence indicate H3K9ac and nuclei, respectively. Bar = 50 μm. (D) Quantification of fluorescence intensity at the two-cell stage (n = 20 per group). (E) Representative immunofluorescent images of H3K9ac at the four-cell stage. Green and blue fluorescence indicate H3K9ac and nuclei, respectively. Bar = 50 μm. (F) Quantification of fluorescence intensity at the four-cell stage (n = 18 per group). The data are from three independent experiments and are means ± SEM. Values with different superscripts differ significantly (P < 0.05). Con, control; CH, chaetocin; T, TSA.