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. 2021 Oct 19;12(11):963. doi: 10.1038/s41419-021-04230-5

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© The Author(s) 2021, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Expression levels of miR-193b-3p in the mouse back skin of different groups. Vehicle means mice injected with agomiR-NC and treated with Vaseline; agomiR-NC + IMQ and agomiR-193 + IMQ means mice injected with agomiR-NC and agomiR-193b-3p, respectively,and then treated with IMQ for 5 consecutive days. histologicalmiR-193b-3p levels were analyzed using qRT-PCR. U6 was used as the internal control. Each bar represents mean ± SEM (n = 8). ^***P < 0.001, compared with the indicated controls. B Representative pictures of the mouse back skins for agomiR-193b-3p injection or agomiR-NC injection with or without IMQ treatment for 5 consecutive days. C PASI scores of mouse back skin lesions in different groups at different time points. PASI scores of mouse back skin lesions were recorded and analyzed daily. Each bar represents mean ± SEM (n = 8). ^***P < 0.001, compared with the indicated controls. D Representative images showing histological feature of skin tissues derived from mouse back injected with agomiR-193 or agomiR-NC in the presence or absence of IMQ treatment for 5 consecutive days. Scar bar of upper panel: 150 µm, scar bar of lower panel: 100 µm. E Quantification data of skin epidermal thickness (upper panel, n = 8) and Ki67-positive cells (lower panel, n = 8) in skin tissues derived from mouse back injected with agomiR-193 or agomiR-NC in the presence or absence of IMQ treatment for 5 consecutive days. ^***P < 0.001, compared with the indicated controls. F Representative blots showing the levels of phospho-STAT3 (Tyr705), phospho-STAT3 (Ser727), phospho-NF-κB p65 (Ser311), IL-1β, etc. in skin tissues derived from mouse back injected with agomiR-193 or agomiR-NC in the presence or absence of IMQ treatment for 5 consecutive days. Tissues were collected at day 6 and protein levels were detected by western blotting. GAPDH was used as a protein loading control. G mRNA levels of psoriasis-related genes and inflammatory genes in skin tissues derived from mouse back injected with agomiR-193 or agomiR-NC in the presence or absence of IMQ treatment for 5 consecutive days. Skin tissues were collected at day 6 and mRNA levels were analyzed using qRT-PCR. GAPDH was used as the internal control. Each bar represents mean ± SEM (n = 8 for each group). ^***P < 0.001, ^**P < 0.01, ^*P < 0.05 compared with the indicated controls.