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. 2021 Oct 19;12(11):963. doi: 10.1038/s41419-021-04230-5

Fig. 6. Function of ERBB4 knock down in keratinocytes.

Fig. 6

A Expression levels of ERBB4 in HaCaT cells transfected with ERBB4 siRNAs or si-CTL. si-ERBB4#1 and si-ERBB4#2 means two siRNAs specifically targeting ERBB4, while si-CTL means control for interference RNAs. Cells were collected 2 days after transfection and mRNA levels were analyzed using qRT-PCR. GAPDH was used as the internal control. Each bar represents mean ± SEM (n = 3). **P < 0.01, *P < 0.05, compared with the indicated controls. B Representative blots showing protein levels of ERBB4 in HaCaT cells transfected with ERBB4 siRNAs or si-CTL. Protein levels were detected by western blotting. GAPDH was used as a protein loading control. C Relative proliferation rate of HaCaT cells transfected with ERBB4 siRNAs or si-CTL. Cell proliferation was analyzed using MTT assay. Each bar represents mean ± SEM (n = 3). ***P < 0.001, compared with the indicated controls. D The mRNA levels of psoriasis-related genes and inflammatory genes in HaCaT cells transfected with ERBB4 siRNAs or si-CTL in the presence or absence of M5 treatment. Cells were collected 2 days after transfection and mRNA levels were analyzed using qRT-PCR. GAPDH was used as the internal control. Each bar represents mean ± SEM (n = 3). ***P < 0.001, **P < 0.01, *P < 0.05, ###P < 0.001 compared with the indicated controls. E Representative blots showing the levels of phospho-STAT3 (Tyr705), phospho-STAT3 (Ser727), phospho-NF-κB p65 (Ser311), and IL-1β in HaCaT cells transfected with ERBB4 siRNAs or si-CTL in the presence or absence of M5 treatment. Protein levels were detected by western blotting. GAPDH was used as a protein loading control.