Figure 2.
Expression of SSX241-49-specific CARs in T cells
(A) Representative expression of the SSX241-49 CARs demonstrated by the co-detection of the scFv using an anti-human Fab antibody and the QBEnd/10 epitope using an anti-CD34 (clone QBEnd/10) antibody in transduced human HLA-A2+ T cells. Histogram analysis shows expression of either the scFv or the QBEnd/10 epitope, and co-expression of scFv and QBEnd/10 epitope is presented by dot plots analysis. (B) Summary of the transduction efficiency of SSX241-49 CARs transduction efficiency (n = 5). (C) Total expansion of untransduced (UnTx), SSX241-49 28ζ transduced, and SSX241-49 BBζ transduced T cells after 14 days of culture (n = 5).