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. 2021 Sep 29;297(5):101229. doi: 10.1016/j.jbc.2021.101229

Figure 5.

Figure 5

Hematopoietic ERα−/−does not influence metabolism and BM responses to HFD. Male and female bone marrow transplants (BMTs) were generated with either ERα−/− or WT BM. Animals were placed on either ND or HFD. Donor and recipient sex were the same in these studies. A, GTT was performed at 10 weeks of diet. While repeated-measures analysis of glucose responses revealed a weak effect of genotype, overall when diet and sex conditions were tested, there was no significant pairwise difference because of ERα−/−. Flow cytometry of GWAT and IWAT ATMs (B) likewise showed significant differences by sex and diet but no influence of ERα−/−. GWAT adipose tissue T-cells (C) only demonstrated a difference in genotype in FoxP3+ T cells in males. Pre–granulocyte and macrophage (pre-GM) progenitors in bone marrow (D) significantly varied by diet and genotype. Consistent with this, BM myeloid methylcellulose colony-forming units (CFUs) (E) showed increased G and M myeloid colonies in ERα−/− BM of both sexes; however, this was not significantly different in animals challenged to HFD. N = 4 to 8 per group. Data shown as average ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.005, and ∗∗∗∗p < 0.001. #p < 0.05, ##p < 0.01, ###p < 0.005, and ####p < 0.001 marks differences between ND and HFD of the same sex and donor group. ATM, adipose tissue macrophage; BM, bone marrow; ERα−/−, ER-deficient alpha; GTT, glucose tolerance test; GWAT, gonadal white adipose tissue; HFD, high-fat diet; IWAT, inguinal white adipose tissue; ND, normal diet.