Figure 6.

Male and female BM has exaggerated in vitro myeloid stimulation with palmitate after testosterone exposure. To understand the impact of palmitate and/or testosterone, myeloid methylcellulose CFU assays were plated from WT male (A) and female mice (B). There was a stepwise increase in myeloid colonies in males with the addition of palmitate and testosterone. Total cell number counts after 7 days (C) did show a significantly higher number of cells in male and female plates treated with a combination of testosterone and palmitate. N = 9 to 15 for CFU assays. Data shown as average ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.005, and ∗∗∗∗p < 0.001. BM, bone marrow; CFU, colony-forming unit.