Figure 7.

Male AR^tfmanimals on HFD have attenuated metainflammation. WT and AR^tfm male and female mice were placed on ND or HFD starting at 6 weeks of age. Weight gain (A), GTT (B), ITT (C), and fed glucose levels (D) were all significantly worsened in male WT mice and improved to the level of female animals by AR deficiency. There was no effect of AR^tfm in female mice. GWAT weights (E) were significantly different by genotype (p = 0.017), but there was no post hoc difference in males or females. Liver weights (F) were highest in WT males and normalized by AR^tfm. Both circulating Ly6c^hi monocytes (G) and CD11c⁺ ATMs (H, immunofluorescence [caveolin, green and Mac 2, magenta], I) were highest in WT males. J, AR^tfm reduced myeloid and total granulocyte and macrophage colonies in HFD males. N = 4 to 8 per group of mice. Data shown as average ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.005, and ∗∗∗∗p < 0.001. AR, androgen receptor; GTT, glucose tolerance test; GWAT, gonadal white adipose tissue; HFD, high-fat diet; ITT, insulin tolerance test; ND, normal diet.