Fig. 4.

Evaluation of autophagic activity following SREBP1c silencing and OA administration

Cells were transfected with siRNA specific for SREBP1c and then treated with OA:BSA complex (0.5 mM) for 24 h to divide into following groups; i) control, ii) OA, iii) SREBP1c siRNA, iv) SREBP1c siRNA + OA. Representative BODIPY/LAMP1 co-stained confocal microscopic images from each group. Cells were fixed, and then stained for BODIPY (green), LAMP1 (red) and nuclei by DAPI (blue). The graph shows quantification of the numbers of lipid droplet co-localized with LAMP1 per cell as explained in Methods section (A). Protein expressions of each group were measured by western blotting following the normalization to β-actin against Beclin 1 (B) and LC3 antibodies (C). Scale bar = 10 μm

Data are expressed as mean ± S.D.

**p < 0.01, and *p < 0.05 vs. control,

^#p < 0.05 vs. OA, (n = 3). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)