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. 2021 Oct 6;12:743993. doi: 10.3389/fpls.2021.743993

FIGURE 4.

FIGURE 4

Relative expression of genes involved in the synthesis of precursor PPP (phytyl pyrophosphate), through the recycling of free phytol (A) and MEP pathway (B), and of precursor HGA (homogentisate) through the SK pathway (C), in the flavedo during fruit maturation of grapefruit (C. paradisi), lemon (C. limon), sweet orange (C. sinensis), and mandarin (C. clementine). The genes analyzed were VTE5 (phytol kinase), VTE6 (phytyl-P kinase), DXS1 and DXS2 (1-deoxy-D-xylulose-5-phosphate synthase 1 and 2), GGPPS1 and GGPPS6 (geranylgeranyl pyrophosphate synthase 1 and 6), GGDR (geranylgeranyl diphosphate reductase), TAT1 (tyrosine aminotransferase), and HPPD (4-hydroxyphenylpyruvate dioxygenase). Maturation stages correspond to immature green (IG), mature green (MG), breaker (Br), and mature (M). The data are mean ± SE of at least three replicates (Supplementary Table 11). Different letters indicate significant mean differences among maturation stages for each fruit specie (Tukey’s test, p ≤ 0.05).