FIG. 2.

Specificity of the indicated PCRs. (A) DNAs (100 ng each) of BHV-1.1 LA (lanes 1), BHV-1.2 K22 (lanes 2), gE-negative vaccine virus strain Difivac (lanes 3), and PRV (lanes 4) were used as templates. (B) Results of the indicated PCRs with DNA from BHV-5 N569 compared to the results obtained with DNA from BHV-1.1 LA. BHV-5 is not detected by the gC- and gE-specific PCR. The PCR products (10 μl) were separated in a 1.0% agarose gel. The sizes of the amplified products are indicated (lane M, 1-kb ladder as a size marker).