FIG. 5.

Different copy numbers of gB and gE were found in individual blood samples (from animals BO-1 to BO-8 and EC-1 to EC-8). The amplification products obtained by the indicated PCRs (A to E; the size of each PCR fragment is indicated to the right) were blot hybridized with the respective internal probes. The X-ray films were exposed for 2 h (A), 1 h (B), 20 h (C), 7 h (D), and 68 h (D). It can be seen that the positive samples from animals in herd BO as well as the samples from animals EC-1, EC-3, and EC-6 contained 10- to 100-fold larger amounts of gB than gE.