TABLE 1.

Sequences of synthetic oligonucleotide primers and their locations in the respective glycoprotein genes

Oligonucleotide	Sequence	Primer location (nucleotide position)	PCR product	PCR product length (bp)
gB-1a	5′-TACGACTCGTTCGCGCTCTC-3′	883–902
gB-2	5′-GGTACGTCTCCAAGCTGCCC-3′	1341–1360	gB1/2	478
gBN-1	5′-TCTCGACCGGGGACATTATC-3′	899–918
gBN-2	5′-GCCTCTTCGATCACGCAGTC-3′	1264–1283	gBN1/2	385
gC-1b	5′-AGGAGCGCAAGTGGATGCTCTG-3′	563–581
gC-2	5′-GTAGCCGTTGCGGAACCAGTGC-3′	1068–1087	gC1/2	527
gE-1c	5′-GCTTCGGTCGACACGGTCTT-3′	501–520
gE-2	5′-CTTTGTCGCCCGTTGAGTCG-3′	746–765	gE1/2	265
gEN-1	5′-CGCGCCCGTCTTTCTCCCA-3′	536–554
gEN-2	5′-CACGTCGGTGAAGCACTCG-3′	655–674	gEN1/2	139

^aBHV-1.1 Cooper, accession no. M21474. 

^bBHV-1.1 Cooper, accession no. M27491. 

^cBHV-1.2 FM, accession no. U06934.