TABLE 2.
PCR revealed different copy numbers of gB and gE in PBLs
| Animal | No. of cells with one BHV-1 DNA copy by PCR witha:
|
ELISA resultb | SNT dilutionc | |
|---|---|---|---|---|
| gB-1–gB-2 | gE1–gE2 | |||
| EC-1 | 1 × 104 | 1 × 106 | NTd | 1:64 |
| EC-2 | 1 × 102 | 1 × 102 | NT | 1:128 |
| EC-3 | 5 × 102 | 5 × 104 | NT | 1:32 |
| EC-5 | 1 × 103 | 1 × 103 | NT | 1:128 |
| EC-6 | 1 × 103 | 1 × 105 | NT | 1:128 |
| EC-7 | 1 × 104 | 1 × 104 | NT | 1:32 |
| EC-8 | 1 × 104 | 1 × 104 | NT | 1:64 |
| BO-1 | 1 × 103 | 1 × 105 | Pos ? | NT |
| BO-2 | 1 × 105 | Neg | Neg | NT |
| BO-3 | 1 × 103 | 1 × 105 | Pos | NT |
| BO-4 | 1 × 105 | 1 × 106 | Neg | NT |
| BO-5 | 1 × 104 | 1 × 105 | Pos | NT |
| BO-6 | 1 × 105 | Neg | Neg | NT |
| BO-7 | 5 × 102 | 5 × 104 | Pos | NT |
| BO-8 | 1 × 105 | 1 × 106 | Neg | NT |
a
The calculation was performed after Southern blot hybridization, as described in Materials and Methods. Neg, negative result.
b
BHV-1-specific ELISA (Screening [IDEXX GmbH] and Checkit Trachitest-screeningtest [Bommeli AG, Hoechst Roussel Vet.], performed according to the instructions of the manufacturer. Pos, positive result; Pos ?, doubtful positive result; neg, negative result.
c
SNT, serum neutralization test without complement; with the indicated serum dilution, 100% neutralization of BHV-1.1 was obtained.
d
NT, not tested.