Table 2.
Primer sequences for quantitative real-time polymerase chain reactions used in the present study.
| Gene name | Primer sequences | |
|---|---|---|
| KDM1A (LSD1) | Forward | 5′-CATCATGGTGCAAGAAGAGC |
| Reverse | 5′-ATGTGGGAAAGGCAGACAAG | |
| KDM4B (JMJD2B) | Forward | 5′-CTGAAATGCGTGTACTGCCG |
| Reverse | 5′-CGTTGCGGTTCTTGGTGATG | |
| KDM4C (JMJD2C) | Forward | 5′-GCTCGATTTTCCACAGCCTC |
| Reverse | 5′-CAGGGTCGGCCACATATTCA | |
| KDM5B (JAR1D1B) | Forward | 5′-GTACTGTGAAGGACGCACCA |
| Reverse | 5′-TAGCACCGTTTACAGGCTGG | |
| KDM6B (JMJD3) | Forward | 5′-CACCCACTGTGGTCTGTTGT |
| Reverse | 5′-TGTCTCCGCCTCAGTAACAG | |
| HDAC1 | Forward | 5′-CGGTGCTGGACATATGAGAC |
| Reverse | 5′-TGGTCCAAAGTATTCAAAGTAGTCA | |
| HDAC2 | Forward | 5′-CCAGATGTTCTGGCATCCTC |
| Reverse | 5′-ACAGCCCCTGTTGTCCTGT | |
| HDAC3 | Forward | 5′-TGGCATTGACCCATAGCCTG |
| Reverse | 5′-TGCATATTGGTGGGGCTGAC | |
| HDAC4 | Forward | 5′-CGGAAGCATGTGTTTCTGCC |
| Reverse | 5′-TTCTCCATGGAACGGACAGC | |
| HDAC5 | Forward | 5′-TCTTGTCGAAGTCAAAGGAGC |
| Reverse | 5′-GAGGGGAACTCTGGTCCAAAG | |
| HDAC6 | Forward | 5′-TGGCTATTGCATGTTCAACCA |
| Reverse | 5′-GTCGAAGGTGAACTGTGTTCCT | |
| HDAC7 | Forward | 5′-GAACAGTCCATCCCAACAGC |
| Reverse | 5′-GGCTCCTTCCGTCTCCAG | |
| HIF-1α promoter | Forward | 5′-CACCCCCATCTCCTTTCTCT-3′ |
| Reverse | 5′-GGGTTCCTCGAGATCCAATG-3′ | |
| HIF-2α promoter | Forward | 5′-GAAGTGCGGAGGCAGGAG-3′ |
| Reverse | 5′-GGCAGCCCACTTTAAAAACTC-3′ | |
| GAPDH | Forward | 5′-GAAGAGCCAAGGACAGGTAC |
| Reverse | 5′-CAACTTCATCCACGTTCACC | |
KDM Lysine de-methylase, HDAC Histone de-acetylase, LSD1 Lysine-specific demethylase 1, JMJD Jumonji domain-containing proteins, HIF hypoxia-inducible factor, GAPDH Glyceraldehyde 3-phosphate dehydrogenase.