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. 2021 Oct 6;12:676182. doi: 10.3389/fgene.2021.676182

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Copyright © 2021 Unni, Reddy, Pal, Sagi and Galande.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Types of Hvul_YAP expressing cells in Hydra. Immunofluorescence assay of Hvul_YAP performed using anti-CheYki antibody on macerated cells at 60×. (A) This panel shows cell types based on signal intensity or YAP expression level in cells. Blue arrow represents cells with high YAP expression, yellow arrow represents cells with medium YAP expression, cells with a green arrow represents low YAP expression. White arrow indicates extra-nuclear staining in nematocysts. (B) This panel depicts cell types based on the cellular arrangement. Orange arrows represent cells with duplet or quadruplet arrangement and red arrow represents cells arranged linearly. Red: YAP & Blue: Nucleus (Magenta indicates merged image). Immunofluorescence assay using the anti- Hvul_YAP antibody of macerated cells at 60×. The red fluorescent dye shows Alexa 594 staining of YAP and the blue dye shows DAPI staining of nucleus (Scale bar, 20 µm).