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. 2021 Oct 6;12:676182. doi: 10.3389/fgene.2021.676182

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Copyright © 2021 Unni, Reddy, Pal, Sagi and Galande.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Disruption of YAP-TEAD interaction in Hydra leads to an increase in budding kinetics. The YAP-TEAD interaction in Hydra was targeted using verteporfin (Vp) and the budding rate of the polyps were assayed against DMSO treated control polyps by calculating average buds per Hydra for the period of 10 days post treatment. (A) Vp treatment (5 µM) led to significant increase in the budding rate in Vp treated polyps as compared to the DMSO treated control polyps from day 3–10 (Refer Supplementary Table S2 for statistical data). 48 polyps were scored per set per experiment and total of three biological replicates were performed for each set. (B) Bud detachment assay was performed by calculating cumulative buds detached on each day. This experiment showed no change is Vp vs DMSO treated polyps indicating that increased budding rate is a function of cell proliferation and not bud detachment rate.