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. 2021 Oct 6;9:734818. doi: 10.3389/fcell.2021.734818

FIGURE 1.

FIGURE 1

TEOA induced relative selective cytotoxicity of human pancreatic cancer cells. (A) HPDE6-C7, SW1990, and PANC1 cells were treated with various concentrations of TEOA (0–60 μM) for 12 h. The cell viability was detected using CCK-8 assays (left). LDH activities of drug-treated cells were measured by LDH cytotoxicity assay (right). (B) Cells were treated with 30, 35, 40 μM TEOA for 4 h, 8 h, 12 h and photographed by microscope. Scale bars 20 μm. The arrows were used to mark the vacuolation changes. (C) Cells were treated with TEOA at 0, 25, 30, 35 μM and cell proliferation was detected by EdU assay after 12 h. (D) Cell proliferation ability with long-term TEOA treatment was estimated by the colony formation assay, and colonies were analyzed on the right (★p < 0.05; ★★p < 0.01; ★★★p < 0.001, between groups).