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. 2021 Oct 6;9:734818. doi: 10.3389/fcell.2021.734818

FIGURE 4.

FIGURE 4

TEOA induced ROS accumulation and oxidative stress in pancreatic cancer cells. (A–D) To assess intracellular ROS production, TEOA treated SW1990 and PANC1 cells were loaded with DCFH-DA probe for 30 min followed by confocal laser microscope (A,B, Scale bars: 25 μm) and flow cytometry measurement (C,D). (E) SW1990 cells were treated with TEOA at indicated concentrations for 8 h, and expression of oxidative stress-related proteins catalase and MnSOD were detected by western blot. GAPDH was used as a loading control. (F) Catalase and MnSOD were quantified by normalizing to GAPDH. (G,H) Similarly, catalase and MnSOD expression of TEOA-treated PANC1 cells were determined by western blot, and the quantitative results were shown on the right (★p < 0.05; ★★p < 0.01, versus control).