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. 2021 Oct 6;9:734818. doi: 10.3389/fcell.2021.734818

FIGURE 7.

FIGURE 7

The effects of autophagy activity on TEOA-induced cytotoxicity. (A) Control and ATG5 shRNA-transfected SW1990 cells were treated with 0, 30, 35 μM TEOA. The cell viability was evaluated using CCK-8 assay after 12 h. (B) SW1990 cells were treated with TEOA (32 μM) alone or combined with CQ (25 μM), 3MA (5 mM), respectively. CCK-8 test was used to assess cell viability. (C) Control and ATG5 shRNA-transfected SW1990 cells were treated with TEOA (45 μM) for 8 h, (D) SW1990 cells were treated with TEOA alone or combined with CQ (25 μM), respectively, and expression of LC3 were detected by western blot, GAPDH was used as a loading control. (E) Cells were exposed to TEOA (30 μM) with or without rapamycin (0.4 μM) for 12 h, and then cell viability was measured using the CCK-8 assay. (F) SW1990 cells were pretreated with or without EBSS for 4 h and then incubated with TEOA for 12 h. Cell viability was measured by CCK-8 assay (★p < 0.05; ★★p < 0.01, versus control).