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. 2021 Oct 6;9:734818. doi: 10.3389/fcell.2021.734818

FIGURE 8.

FIGURE 8

NAC reduced the cytotoxicity of TEOA and inhibited autophagy by restoring mTOR/p70S6k signaling pathway in SW1990 cells. (A) SW1990 cells were treated with TEOA alone or in combination with 0.5 mM NAC, and then ROS levels were measured by flow cytometry after 8 h. (B) Statistical analysis of relative ROS level and the results were presented as the mean ± SD from three independent experiments. (C) SW1990 cells were treated with TEOA alone or in combination with 0.5 mM NAC. After 12 h, images were captured using optical microscope (20×). (D) SW1990 cells were treated with TEOA at various concentrations alone or in combination with 0.5 mM NAC for 12 h and cell viability was detected by CCK-8 assay. (E) After incubating with individual TEOA or combination with NAC for 8 h, protein expression of mTOR / p70s6k signaling pathway was determined by western blot (★p < 0.05; ★★p < 0.01; ★★★p < 0.001, versus control).