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. 2021 Oct 6;12:708967. doi: 10.3389/fphar.2021.708967

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Copyright © 2021 Shi, Zheng, Pan, Zhang, Zhuang and Fu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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GA inhibited the EMT and metastasis of HCC cells. HepG2 (A) and Bel-7402 (C) cells were treated with 80 μM for 48 h, and wound-healing migration was assayed. The HepG2 (B) and Bel-7402 (D) cells treated with 80 μM GA and invasion was examined. (E,G) The mRNA expression of E-cadherin, Vimentin, N-cadherin and Fibronectin in GA-treated HepG2 cells (E) and Bel-7402 cells (G) were measured by q-RT-PCR. (F,H) The mRNA expression of ZEB1, ZEB2, SNAI1, SNAI2, TWIST in GA-treated HepG2 cells (F) and Bel-7402 cells (H) were measured by q-RT-PCR. Data are presented as mean ± SEM. *, p < 0.05; **, p < 0.01; ***, p < 0.001; vs NC.