Table 1.
Demographic, diagnostic, and laboratory data and stem cell transplantation details of the four children with autism spectrum disorder (ASD).
| Case #1 | Case #2 | Case #3 | Case #4 | |
|---|---|---|---|---|
| Gender | Male | Male | Male | Female |
| Age at time of diagnosis of autism | 4 y | 3 y | 4 y | 2 y |
| Diagnostic details | F84.0 (ADOS = 13) + F90.0 + F79 neuropsychiatrist |
F84.0 (CARS = 28.5) + F82 neuropsychiatrist |
F84.0 (CARS = 38.5) + F90.0 + F80.0 pediatric psychiatrist |
F84.1 (CARS = 25) + F80.1 + F71 pediatric neurologist |
| Therapy before and/or after autologous SCT | speech therapy, medication |
ABA, speech therapy, medication, nut. suppl. |
medication, nut. suppl., horse therapy |
ABA |
| Age and body weight at time of first procedure | 7 y2 m, 29 kg | 7 y5 m, 32 kg | 14 y4 m, 74 kg | 3 y8 m, 17.5 kg |
| Bone marrow related details | ||||
| BM aspirate (ml/kg body weight) | 1.55 | 1.40 | 0.81 | 1.42 |
| CD-34 cell concentration in BM aspirate (μl−1) | 302 | 406 | 182 | 330 |
| CD-34 cell concentration in BM concentrate (μl−1) | 600 | 1,040 | 950 | 1,090 |
| CD-45 mononuclear cells in BM aspirate (nl−1) | 11.9 | 31.2 | 12.9 | 23.0 |
| CD-45 mononuclear cells in BM concentrate (nl−1) | 22.4 | 144.0 | 62.1 | 59.4 |
| SC concentrate (ml/kg body weight) | 0.28 | 0.31 | 0.14 | 0.57 |
| Stem cells related details | ||||
| Intrathecal vol. of SC concentrate instillation | 3.0 ml | 4.0 ml | 5.0 ml | 2.5 ml |
| Intrathecal number of CD-34 cells injected | 1.8 x 106 | 4.16 x 106 | 4.75 x 106 | 2.73 x 106 |
| Intravenous vol. of SC-concentrate injected | 5.0 ml | 6.0 ml | 5.0 ml | 7.5 ml |
| Intravenous number of CD-34 cells injected | 3 x 106 | 6.24 x 106 | 4.75 x 106 | 8.18 x 106 |
| ‡SC viability in BM aspirate (%) | 92.9 | 89.1 | 66.0 | 92.6 |
| ‡SC viability in SC concentrate (%) | 87.0 | 90.0 | 57.3 | 90.2 |
ABA, Applied Behavior Analysis Therapy; ADOS, Autism Diagnostic Observation Schedule; ASD, autism spectrum disorder; BM, bone marrow; CARS, Childhood Autism Rating Scale; F71, moderate mental retardation; F79, unspecified mental retardation; F80.0, specific speech articulation disorder; F80.1, expressive language disorder; F82, specific developmental disorder of motor function; F84.0, childhood autism; F84.1, atypical autism; F90.0, Disturbance of activity and attention; m, months; nut. suppl., nutritional supplements; OP, operation/surgery; SC, stem cell; SCT, stem cell transplantation; y, years.
viability as determined 24–36h after harvesting.