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. 2021 Oct 6;12:663695. doi: 10.3389/fimmu.2021.663695

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Copyright © 2021 Ó Maoldomhnaigh, Cox, Phelan, Mitermite, Murphy, Leisching, Thong, O’Leary, Gogan, McQuaid, Coleman, Gordon, Basdeo and Keane

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Lactate reduces TNF and IL-1β production. PBMC were isolated from buffy coats and MDM were adherence purified for 7 days in 10% human serum. Cells were treated with lactate (25 mM) for 3 hours prior to stimulation with Mtb (iH37Rv; MOI 1-10) or LPS (100 ng/ml). The concentrations of IL-1β (A), TNF (B), IL-10 (C) and IL-6 (D) in supernatants were measured by Mesoscale Discovery assay 24 hours after stimulation with Mtb or LPS (n=4). Statistical significance was determined using two-way ANOVA with Sidak’s multiple comparison test; *P < 0.05, **P < 0.01.