Figure 2.

Proportion of infected cells and parasite load after TLR2 and TLR4 neutralization. Monocytes from PBMCs of CL patients (N=9) and HS (N=4) were infected with L. braziliensis (5:1) for 2 or 48 hours. Numbers of infected cells after 2 hours (A) and 48 hours (C), as well as the number of internalized parasites after 2 hours (B) and 48 hours (D) were quantified by optical microscopy. Data are representative of median values of infected cells and internalized parasites After 72 hours, RPMI medium was replaced with Schneider’s medium, and the number of viable promastigotes was estimated after 3 days (E). Data are representative of median numbers of viable parasites. All p values were obtained using the Wilcoxon signed-rank test, *p<0.05, **p<0.01, ***p<0.001.