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. 2021 Oct 6;12:706510. doi: 10.3389/fimmu.2021.706510

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Copyright © 2021 Carneiro, Dórea, Oliveira, Guimarães, Brodskyn, Carvalho and Bacellar

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The TNF production after stimulation with leishmania soluble antigen (SLA) and TLR2 and TLR4 neutralization. Monocytes from CL patients (n = 10) were treated or not with anti-TLR2 and anti-TLR4 antibodies. Cells were stimulated with SLA for 8 hours. (A) The positive florescence in the population cells were obtained by the strategy using FMO (Florescence Minus One). The frequency of cells expressing TNF in CD14+ cells (B) was performed by the flow cytometry technique. All p values were obtained using Wilcoxon’s statistical test **p<0.01.