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Effects of TLR2 and TLR4 neutralization on cytokine/chemokine production in biopsied cells from CL patient lesions. Cells from the lesions of patients with CL (n=10) were obtained and cultured for 48h in the presence or absence of anti-TLR2 and anti-TLR4. TNF (A), IL-1β (B), CXCL9 (C) CXCL10 (D) and IL-10 (E) were measured in culture supernatants by ELISA. Data are representative of median cytokine/chemokine levels. All p values were obtained using Wilcoxon signed-rank test; **p<0.01.
