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. 2021 Oct 6;12:754106. doi: 10.3389/fimmu.2021.754106

Figure 6.

Figure 6

Effects of blockade of IL-4 or IFN-γ on macrophage differentiation and cytokines production from iNKT cells. (A) The levels of mRNA encoding genes related to a pro-inflammatory macrophage phenotype (Tnf, Il1b, Il6, Nos2, and Ifng) and genes related to a reparative macrophage phenotype (Mrc1, Retnla, Il4, and Il13) in macrophages co-cultured with iNKT cells. Macrophages co-cultured with iNKT cells in the presence of α-GalCer were incubated for 72 h with an IL-4- (5 μg/mL) or IL-13-neutralizing antibody (5 μg/mL). Data are expressed as the mean ± SD (four mice per group, representative data from two independent experiments). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. (B) The levels of mRNA for Il4 and Ifng from iNKT cells. NKT cells co-cultured with macrophages in the presence of α-GalCer were incubated for 72 h with IL-4 (5 μg/mL) or IL-13 neutralizing antibody (5 μg/mL). Data are expressed as the mean ± SD (four mice per group, representative data from two independent experiments). *P < 0.05, **P < 0.01, ****P < 0.0001.