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. 2021 Oct 6;11:733745. doi: 10.3389/fonc.2021.733745

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Copyright © 2021 Yang, Zhou, Zeng, Zhou, Wang, Li, Zhu, Guan and Chai

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Circ-0002570 expression was distinctly increased in GC. (A) The Heatmap was drawn to show the differentially expressed circRNAs in GC tissues by analyzing the GSE31210. (B) Sanger sequencing was applied to demonstrate the back splicing junction. (C) RT-PCR and gel electrophoresis were performed to check the existence of circ-0002570. Circ-0002570 can only be amplified in cDNA. (D) The relative levels of FAM73A and circ-0002570 were examined by RT-PCR after treatments with RNase R or mock in AGS and SGC7901 cells. (E) RT-PCR for the expression of circ-0002570 in 24 pairs of GC specimens and non-tumor specimens. (F) The levels of circ-0002570 in six GC cells and GSE-1 cells were determined by RT-PCR. *p < 0.05, **p < 0.01, ***p < 0.001. ns, no significant.