Table 1.
Cryo-EM data collection, 3D image processing and model refinement statistics
| Data collection parameter |
PfK5ΔL6-MD nucleotide state |
|
|---|---|---|
| No nucleotide | AMPPNP | |
| Data Collection and 3D Image Processing | ||
| Magnification | 160,000 | 160,000 |
| Voltage (kV) | 300 | 300 |
| Electron dose (e−/Å2) | 58 | 58 |
| Pixel size (Å) | 1.39 | 1.39 |
| Number of Images | 1320 | 335 |
| Data Collection Strategy | Manual and automated | Manual |
| Starting number of particles | 48,165 | 37,843 |
| Final particle number (after particle symmetry expansion) | 73,684 | 206,666 |
| Box size (pixels) | 432 | 432 |
| C1 reconstruction resolution (Å) | 4.5 | 4.8 |
| Asymmetric unit refine resolution (Å) | 4 | 4.4 |
| Model Refinement | ||
| Global Cross-correlation | ||
| Homology model | 0.88 | 0.86 |
| Final model | 0.89 | 0.89 |
| MolProbity | ||
| Homology model | 3.46 | 3.46 |
| Final model | 1.13 | 0.98 |
| QMEAN | ||
| Homology model | −2.21 | −2.21 |
| Final model | −0.36 | 0.16 |