TABLE 2.
Identification of HPV genotypes by direct sequencing of MY 09/11 and SPF amplimers and by SPF LiPA in 104 clinical samples (group 1)
| HPV type based on MY 09/11 sequence (n) | No. of isolates for which SPF sequence-based typinga was concordant with MY 09/11 typing | Type(s) determined by the following method when SPF-based typing was initially discordant with MY 09/11 sequence:
|
||
|---|---|---|---|---|
| SPF sequence | Type-specific PCRb | MY 09/11 and SPF LiPA | ||
| 6 (12) | 12 | |||
| 11 (2) | 2 | |||
| 16 (19) | 18 | 31 | 16, 31 | 16, 31 |
| 18 (8) | 7 | 16 | 16, 18 | 16, 18 |
| 30 (1) | 1 | |||
| 31 (9) | 9 | |||
| 33 (7) | 7 | |||
| 35 (6) | 6 | |||
| 39 (1) | 1 | |||
| 45 (3) | 3 | |||
| 51 (1) | 0 | Unknownc | 51 | |
| 52 (9) | 9 | |||
| 53 (1) | 1 | |||
| 55 (1) | 1 | |||
| 56 (5) | 3 | Unknownd | 56 | |
| 45 | 45, 52, 56 | |||
| 57 (1) | 1 | |||
| 58 (6) | 5 | 56 | 56 | |
| 62 (1) | 1 | |||
| 66 (4) | 4 | |||
| 67 (2) | 2 | |||
| 68 (1) | 1 | |||
| 73 (1) | 0 | 53 | 53, 68/73 | |
| 74 (1) | 1 | |||
| MM4 (1) | 1 | |||
| MM7 (1) | 1 | |||
| Total (104) | 97 | 7 | 2 | 7 |
a
HPV genotype based on sequence of the 22-bp SPF interprimer region.
b
Available for HPV-16, -18, -31, and -33, according to Baay et al. (3).
c
The 22-bp sequence from this sample showed a single mismatch to HPV-51.
d
The 22-bp sequence from this sample showed a single mismatch to HPV-56.