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. 2021 Oct 6;12:725572. doi: 10.3389/fmicb.2021.725572

Figure 3.

Figure 3

Francisella tularensis (Ft) LVS fails to replicate in Nlrp3−/− macrophages. Wild-type and Nlrp3−/− macrophages (A,B) were infected with 100 MOI of Ft LVS or (C) with 100 MOI of Salmonella Typhimurium. (A,C) The macrophages were lysed after 4 and 24h of infection, diluted 10-fold and plated on MH-chocolate agar plates for the enumeration of bacterial numbers. (B) Percent cell death was determined by LDH release assay 12 and 24h post-infection. (D) Wild type, Asc−/− or the Caspase1−/− macrophages were infected with 100 MOI of Ft LVS and bacteria were quantitated at 4 and 24h post-infection. (E) Wild-type and Nlrp3−/− primary bone marrow-derived macrophages (BMDMs) isolated from C57BL/6 mice were infected with 10 MOI of Ft LVS and bacteria were quantitated at 4 and 24h post-infection. The data are representative of two–three independent experiments each conducted with three biological replicates. The data were analyzed by ANOVA with the Tukey-Kramer test, and a value of p of 0.05 or less was considered significant. ***p<0.001; ****p<0.0001.