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. 2021 Sep 22;62:100121. doi: 10.1016/j.jlr.2021.100121

Fig. 1.

Fig. 1

Expression of ORMDL family members and antigen-induced signaling in PDMCs isolated from WT and ORMDL3 KO mice. A: Immunoblot of PDMCs isolated from WT and ORMDL3 KO PDMCs. B: Statistical analysis of global ORMDL family members expression in WT (n = 6) and ORMDL3 KO (n = 8). C–E: RT-qPCR quantification of mRNAs encoding ORMDL1 (C), ORMDL2 (D), and ORMDL3 (E) in resting WT (n = 8) and ORMDL3 KO (n = 11) PDMCs. F: Calcium mobilization in antigen (Ag)-activated PDMCs WT (n = 7) ORMDL3 KO (n = 9). G: Antigen-mediated β-glucuronidase release into supernatants of PDMCs WT (n = 25) and ORMDL3 KO (n = 30) activated for 30 min with various concentrations of antigen. Quantitative data are mean ± SEM, calculated from n, which shows numbers of biological replicates of independently isolated PDMCs. P values were determined by unpaired two-sided Student's t test.