Skip to main content
. 2021 Sep 22;62:100121. doi: 10.1016/j.jlr.2021.100121

Fig. 3.

Fig. 3

ORMDL3 in PDMCs limits sphingolipid and leukotriene biosynthesis. A–E: LC-ESI-MS/MS analysis of sphingolipids in resting WT (n = 13) and ORMDL3 KO (n = 12) PDMCs. A: The sum of total sphingosines, C18:1, C18:0, C20:1, and C20:0. B: The values of distinct sphingosines C18:1 and C20:1 and sphinganines C18:0 and C20:0. C: The sum of total ceramide fatty acid chain molecular species derived from C18:1 sphingosine. D: Non-2-hydroxylated ceramide molecular species derived from C18:1 sphingosine. E: Ratio of very long acyl chains molecular species (C22:C26) and long acyl chains molecular species (C14:C20) in resting WT (n = 12) and ORMDL3 KO (n = 12) PDMCs. F–J: UPLC MS/MS analysis of eicosanoids from supernatants of antigen-activated PDMCs WT (n = 13) and ORMDL3 KO (n = 16). F: Prostaglandin PGD2 and thromboxane TXB2 derived from arachidonic acid. G: Leukotrienes derived from arachidonic acid. H: Leukotrienes derived from EPA. I: The total levels of arachidonic acid. J: The total levels of EPA. Quantitative data are mean ± SEM, calculated from n, which show numbers of biological replicates of independently isolated PDMCs. P values were determined by unpaired two-sided Student's t test.