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. 2021 Oct 6;12:756830. doi: 10.3389/fmicb.2021.756830

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Copyright © 2021 Oshkin, Danilova, But, Miroshnikov, Suleimanov, Belova, Tikhonova, Kuznetsov, Khmelenina, Pimenov and Dedysh.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Genome-based phylogeny showing the position of new isolates in relation to other gammaproteobacterial methanotrophs based on the comparative sequence analysis of 120 ubiquitous single-copy proteins. The clade of Methylococcus methanotrophs is highlighted by orange. The tree was constructed using the Genome Taxonomy Database toolkit (Parks et al., 2018), release 04-RS89. The significance levels of interior branch points obtained in maximum-likelihood analysis were determined by bootstrap analysis (100 data re-samplings). Bootstrap values of >70% are shown. The root (not shown) is composed of all genomes available in GTDB for methanotrophs of the genera Methylosinus and Methylocystis. Bar, 0.1 substitutions per amino acid position.