Figure 3.

Analyses of humoral responses induced by TT CovAg vaccine in BALB/c mice

(A) Endpoint titer of TT CovAg vaccinated mice with and without boosts for various routes of administration. Data were acquired at day 49, 14 days after the boost injection date (n = 5, mean ± SEM; two-way ANOVA with Sidak's correction for multiple comparisons; alpha threshold = 0.05). (B and C) Antibody endpoint titers over 195 days for i.n. (B) or i.p. primed (C) mice. The vertical dashed line depicts the boost date (n = 5, mean ± SEM). (D) VSV-S neutralization IC50 values for samples described in (A). (E and F) VSV-S neutralization IC50 values over 195 days for prime only and prime/boost vaccinations given either i.p. (E) or i.n. (F) (n = 5, Log₂ mean ± SEM; ∗p value < 0.05 relative to D14; two-way ANOVA with Dunnet's correction for multiple comparison; alpha threshold = 0.05).