Figure 4.

Proinflammmatory activation of PMN by Stamp2 deficiency. (A) Immmunoblotting of p65-phosphorylation (pp65) vs. total p65 expresssion as an indicator of NF-κB activity (n=6/6). (B) Myeloperoxidase (MPO) secretion into the supernatant of isolated PMN as assessed by ELISA with and without inflammatory stimulation (PMA 100ng/ml; n=5/4/5/4). (C) MPO plasma levels in mice at baseline and subjected to 7 days of I/R as assessed by ELISA (n=4/4/7/8). Graphs show Mean ± SEM. Full blots are shown in Supplemental Figure S5 . Significance was determined by two-way ANOVA followed by Tukey post-hoc test (B, C) or by unpaired Student’s t-test (A). *P < 0.05.