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. 2021 Oct 6;12:701721. doi: 10.3389/fimmu.2021.701721

Figure 5.

Figure 5

PMN depletion rescues impaired LV function in Stamp2-/- mice after I/R. (A) Schematic overview of PMN depletion by i.p. injection of an anti-Ly6G antibody 1 day prior to- and 3 days after subjection to I/R (13). (B) Representative images of Masson’s trichrome-stained cardiac sections of WT- and Stamp2-/- animals 7 days after I/R with (C) assessment of the LV fibrotic areas stained in green/grey (n=8/5). Scale bar=1mm (D) Representative echocardiographic recordings of WT- and Stamp2-/- hearts (A=Apex, R=Aortic root; Diast.=diastole, Syst.=systole). (E) Total ejection fraction (EF, n=6/7/6/7) and (F) change of EF in % to baseline (n=6/7). (G) Total cardiac output (CO, n=6/7/6/7) and (H) change of CO in % to baseline as assessed by echocardiography (n=6/7). (I) Longitudinal strain reduction after subjection to I/R (n=6/7). Baseline echocardiography was directly performed before MI induction and I/R echocardiography at day 7. Graphs show Mean ± SEM. Significance was determined by two-way ANOVA followed by Tukey post-hoc test (E, G) or by unpaired Student’s t-test (C, F, H, I). n.s., not significant.