Study design for census of coral heat tolerance across Florida nurseries. (a) Locations of coral nurseries visited by the R/V Coral Reef II in August and October 2020, and the number of coral individuals tested at each nursery (MML, Mote Marine Laboratory; FWC, Florida Fish and Wildlife Conservation Commission; RR, Reef Renewal; CRF, Coral Restoration Foundation; UM, University of Miami; NSU, Nova Southeastern University; see the electronic supplementary material, table S1 for details). (b) Temperature treatments for CBASS assays. Eight tanks were used to heat one fragment of each coral individual to eight different target temperatures. (c) A dose–response curve modelling Fv/Fm (measured two times per fragment) as a function of maximum treatment temperature was fitted for each coral individual to estimate ED50 (indicated by dashed line) as a metric of thermal tolerance. (Online version in colour.)