TABLE 4.
Titration of three plasma samples using Roche Monitor version 2.0
| Input sample (no. of RNA copies/ml)a | Test dilution | Sample
|
Internal control
|
Virus load (no. of RNA copies/ml) | ||
|---|---|---|---|---|---|---|
| Dilution | OD450 | Dilution | OD450 | |||
| Genotype 1 (7.4 × 106) | 1:1 | 1:625 | 0.500 | 1:25 | 0.958 | 2.47 × 105 |
| 1:10 | 1:125 | 0.535 | 1:25 | 0.441 | 1.08 × 106 | |
| 1:31.6 | 1:125 | 0.398 | 1:25 | 0.598 | 1.77 × 106 | |
| 1:100 | 1:125 | 0.191 | 1:25 | 0.550 | 2.24 × 106 | |
| 1:316 | 1:25 | 0.203 | 1:25 | 0.511 | 1.67 × 106 | |
| 1:1,000 | 1:5 | 0.252 | 1:25 | 0.493 | 1.51 × 106 | |
| 1:3,160 | 1:1 | 0.457 | 1:25 | 0.456 | 2.20 × 106 | |
| 1:10,000 | 1:1 | 0.150 | 1:25 | 0.483 | 1.35 × 106 | |
| Genotype 2 (2.4 × 107) | 1:1 | 1:625 | 0.515 | 1:25 | 0.842 | 2.94 × 105 |
| 1:10 | 1:125 | 0.781 | 1:25 | 0.626 | 1.30 × 106 | |
| 1:31.6 | 1:125 | 0.530 | 1:25 | 0.789 | 2.06 × 106 | |
| 1:100 | 1:125 | 0.214 | 1:25 | 0.734 | 2.21 × 106 | |
| 1:31.6 | 1:125 | 0.150 | 1:25 | 0.731 | 3.89 × 106 | |
| 1:1,000 | 1:25 | 0.191 | 1:25 | 0.814 | 3.32 × 106 | |
| 1:3,160 | 1:5 | 0.314 | 1:25 | 0.525 | 6.92 × 106 | |
| 1:10,000 | 1:5 | 0.192 | 1:25 | 0.418 | 1.43 × 107 | |
| Genotype 3 (1.7 × 107) | 1:1 | 1:625 | 0.563 | 1:25 | 0.865 | 3.16 × 105 |
| 1:10 | 1:125 | 0.869 | 1:25 | 0.571 | 1.63 × 106 | |
| 1:31.6 | 1:125 | 0.447 | 1:25 | 0.351 | 4.33 × 106 | |
| 1:100 | 1:125 | 0.270 | 1:25 | 0.506 | 4.68 × 106 | |
| 1:316 | 1:25 | 0.360 | 1:25 | 0.407 | 5.56 × 106 | |
| 1:1,000 | 1:25 | 0.256 | 1:25 | 0.495 | 9.19 × 106 | |
| 1:3,160 | 1:25 | 0.166 | 1:25 | 0.565 | 1.25 × 107 | |
a
Virus load in test specimens was determined by limiting dilution.