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. 2021 Oct 15;8(6):e1096. doi: 10.1212/NXI.0000000000001096

Figure 3. Heat Maps of CD25 and Anti-inflammatory Cytokine Shift After Anti-CD40L.

Figure 3

(A) Increased CD25/CD3 and CD25/CD4 ratios were seen following treatment. Flow cytometry measured CD25+ vs CD3+ and CD4+ shift after treatment as Z-score heat map. The values on the heat map indicate the ratio of CD25+ cells compared with CD3+ (left) and CD4+ (right). The heat map indicates the change over time in individual patients in the high-dose group (15 mg/kg). (B) IL10/MCP1 and IL10/IL17 were increased following treatment in the same patients. Cytokines in PBMC culture supernatant were measured. Quantity of anti-inflammatory IL10 and proinflammatory cytokine MCP1 and IL17 was measured, and their ratios are presented as Z-score heat map. The ratios are derived by dividing the concentration of IL10 by the concentration of MCP1 (left) or the concentration of IL10 by the concentration of IL17 (right). Across all patients and time points, IL10 ranged between 197 and 565 pg/mL, MCP1 ranged between 5,280 and 15,627 pg/mL, and IL17 ranged between 54 and 1,138 pg/mL. The heat maps indicate the mean change over time in the high-dose group (15 mg/kg). MCP1 = monocyte chemoattractant protein-1; PBMC = peripheral blood mononuclear cell.