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. 2021 Oct 20;19(10):e06846. doi: 10.2903/j.efsa.2021.6846

Table 5.

Batch‐to‐batch analysis of antinutritional factors, phenolics and cyanogenic glycosides in the NF

Parameter (unit) Batches Method of analysis
#1 #2 #3 #4 #5
Lectin (HAU/g) 120 120 120 120 120 Lectin testing by haemagglutination (TES‐AC358)
Phytic acid (mg/g) 13.3 12.6 12.8 11.1 13.4 Phytic acid (PHYT_S)
Trypsin inhibitors (TIU/mg) 5.88 4.35 5.41 2.91 3.55 Trypsin inhibitor (TRYP_IN_S)
Tannins


  • Total phenolic content (mg/g dw)

  • Tannins (condensed) (μg/g dw)

 1.52
5.85 1.02
8.59 1.01
6.81 1.08
6.46 0.97
6.84
  • –

    Folin–Ciocalteu spectrophotometric assay (gallic acid equivalents)

  • –

    DMAC spectrophotometric assay (procyanidin A2 equivalents)
Cyanogenic glycosides (μg/g) < LOD < LOD < LOD < LOD < LOD LC–MS/MS

dw: dry weight; HAU: Haemagglutination Units; TIU: Trypsin Inhibitor Units; DMAC: dimethylaminocinnamaldehyde; LOD: limit of detection of 5 ppb; LC–MS/MS: liquid chromatography with tandem mass spectrometry.