Figure 1.

An integrated approach for analyzing the roles of Insm1, Neurod1, and Pax6 in pancreatic endocrine cell development. Control and KO embryos were collected at either E15.5 or E18.5. GFP-positive cells from E15.5 pancreata were FACS sorted and collected for RNA-Seq, followed by differential expression, Metascape, and differential splicing analyses. Pancreata from E18.5 embryos were fixed in PFA, sections stained for nuclear GFP signal, endocrine hormones, Ki67 and TUNEL reporter, and marked cells were subsequently quantified. Schematic was created with BioRender.com.