Figure 6.

Insm1, Neurod1, and Pax6 regulate alternative splicing in pancreatic endocrine cells. (A) Many important RNA binding proteins (RBPs) are dysregulated in in Insm1, Neurod1, and Pax6 KOs. Bar graph of the log₂ fold change of a subset of dysregulated RBPs. Asterisks indicate P-values of *<0.05, **<0.01, ***<0.001. (B) Venn diagram demonstrates overlaps between differentially spliced genes in Insm1, Neurod1, and Pax6 KO RNA-Seq datasets. Examples of alternatively spliced genes are highlighted with a box insert. Differential splicing events between the knock-out and control groups were identified by performing JunctionSeq analysis on the RNA-Seq datasets. A cutoff of P-adj. value ≤0.01 and log₂ fold change ≥|1| was used.