Figure 3.

Changes in AR and AR-V7 expression levels after subjection to androgen deprivation therapy in LNCaP cells. LNCaP cells in a medium containing 10% fetal bovine serum (FBS), LNCaP cells in a medium containing 10% CS-FBS and LNCaP-LA cells in a medium containing 10% CS-FBS were incubated for 48 h before harvesting for (A) western blotting and (B and C) reverse transcription quantitative PCR. Values were expressed as the means ± standard deviation (n=4). *P<0.05 vs. LNCaP; **P<0.01 vs. LNCaP. AR, androgen receptor; AR-V7, androgen receptor splice variant 7; CS-FBS, charcoal-stripped FBS; LA, low androgen.