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. 2021 Oct 18;30:09636897211052975. doi: 10.1177/09636897211052975

Figure 4.

Figure 4.

M2 macrophages promoted adipogenic differentiation of 3T3-L1 preadipocytes in vitro. (A) The general morphology of M0, M2 and FTY720-treated M2 RAW264.7 cells under a light microscope. Scale bars = 120 µm. (B) Relative mRNA expression of M2 macrophage markers (CD206, Arg-1, Fizz-1) in M0, M2 and FTY720-treated M2 macrophages, by using RT-PCR. The general morphology of 3T3-L1 cells treated with or without FTY720-M2 CM in adipogenic medium (C) and Oil Red O staining identification (D). Scale bars = 120 µm. (E) Triglyceride content was measured after adipogenic induction of 3T3-L1 cells treated with or without FTY720-M2 CM. (F) Relative mRNA expression of adipogenic related genes PPARγ, FABP4, C/EBP-α, and Adipoq in 3T3-L1 cells, matured 3T3-L1 adipocytes cells and matured 3T3-L1 adipocytes cells treated with FTY720-M2 CM.