Table 2.
Inhibition effects of different WIL on enzymatic hydrolysis
| Lignin type | Enzyme | Substrate | Enzymatic hydrolysis | Hydrolysis yield | Refs. |
|---|---|---|---|---|---|
| EOL-LP, EOL aspen and KPL | Cellic CTec 2 | DASG | pH = 4.8, 50 °C, 150 rpm, and 72 h | Decreased from 27.0 to 20.0% | [66] |
| EOL-LP | Novozyme 22C | OPSG and OPLP | 2% (w/v), pH = 4.8, 50 °C, 150 rpm and 72 h | Decreased from 49.3% and 41.2% to 42.0% and 38.1% | [104] |
| EOL-LP | Celluclast and Novozyme 188 | Avicel | 2% (w/v), pH = 4.8, 50 °C, 150 rpm and 48 h | Decreased by 67.8–86.2% | [98] |
| HP | Celluclast and Novozyme 188 | Avicel | 2% (w/v), pH = 4.8, 50 °C, 150 rpm and 48 h | Decreased by 23.0–76.0% | [98] |
| HEL, KL and SEL | Celluclast and Novozyme 188 | Avicel | 2% (w/v), pH = 4.8, 45 °C and 200 rpm | Decreased by 10.0%, 13.0% and 23.0%, respectively | [101] |
| PTL and CEL from steam pretreated poplar | Spezyme CP and Novozyme 188 | Avicel | 5% (w/v), pH = 4.8, 50 °C, 150 rpm and 48 h | Decreased by 8.6% and 11.0%, respectively | [75] |
| PTL and CEL from OPLP | Spezyme CP and Novozyme 188 | Avicel | 5% (w/v), pH = 4.8, 50 °C, 150 rpm and 48 h | Decreased by 23.0% and 25.0%, respectively | [75] |
| EL from OPSG | Novozym 22C | OPSG | 2% (w/v), pH = 4.8, 50 °C, 150 rpm and 72 h | Improved from 72.6 to 77.7% | [105] |
| EL from OPSG | Novozym 22C | OPSG and OPLP | 2% (w/v), pH = 4.8, 50 °C, 150 rpm and 72 h | Increased from 49.3 to 68.6% and from 41.2 to 60.8%, | [104] |
| Liquid hot water treated alkali lignin | Commercial cellulase | filter paper and lignin mixed | 5% (w/v), pH = 4.8, 50 °C, 150 rpm and 72 h | Little inhibition effect | [110] |
| EL from OPSG | Cellic CTec 2 | Cellulose | 2% (w/v), pH = 4.8, 50 °C, 180 rpm and 72 h | No inhibition effect | [103] |
EL extractable lignin, EOL-LP organosolv ethanol lignin from Lodgepole pine, EOL aspen ethanol organosolv lignins from aspen, KPL kraft pine lignin, HP organosolv ethanol lignin from hybrid poplar, HEL hardwood ethanol organosolv lignin from Lignol, KL kraft lignin from Aldrich, SEL softwood ethanol organosolv lignin from lignol, PTL protease treated lignin, CEL cellulolytic enzyme lignin, OPSG organosolv pretreated sweetgum, OPLP organosolv pretreated lodgepole pine, GLMP Green liquor pretreated masson pine, MP Masson pine, DASG Dilute acid pretreatment switchgrass