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. 2021 Oct 12;22(6):828. doi: 10.3892/ol.2021.13088

Figure 1.

Figure 1.

Effect of NACC1-knockdown on NPC cell proliferation. (A) mRNA and (B) protein expression levels of NACC1 in the human normal nasopharyngeal epithelial NP69 cell line and NPC cell lines including SUNE-1, C666-1, 5-8F and 6-10B. *P<0.05, **P<0.01 and ***P<0.001 vs. NP69. (C) mRNA and (D) protein expression levels of NACC1 in control SUNE-1 cells or cells transfected with the indicated shRNAs. (E) Cell proliferation of control SUNE-1 cells or cells transfected with the indicated shRNAs was detected by MTT assay. (F) Representative colony formation assay of control SUNE-1 cells or cells transfected with the indicated shRNAs. (G) Protein expression levels of Ki67 and PCNA in SUNE-1 cells were measured by western blotting. **P<0.01 and ***P<0.001 vs. shRNA-NC. NPC, nasopharyngeal carcinoma; NACC1, nucleus accumbens-associated protein 1; shRNA, short hairpin RNA; NC, negative control; PCNA, proliferating cell nuclear antigen.