Figure 4.

Activation of AKT blocks the effect of NACC1-knockdown on nasopharyngeal carcinoma cell migration and invasion. (A) Representative wound healing assay and quantitative analysis for SUNE-1 cells in different groups (magnification, ×100). (B) Representative Transwell assay and quantitative analysis for SUNE-1 cells in different groups (magnification, ×100). Protein expression levels of (C) MMP2 and MMP9, and (D) E-cadherin, N-cadherin, Vimentin and Snail in SUNE-1 cells were measured by western blot assay. ***P<0.001 vs. shRNA-NC. ^#P<0.05, ^##P<0.01 and ^###P<0.001 vs. shRNA-NACC1. NACC1, nucleus accumbens-associated protein 1; shRNA, short hairpin RNA; NC, negative control.