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Effect of KLK6 on the stem cell-like properties of the HGC-27 cells. (A) Microsphere formation ability was observed using a microscope. Scale bar, 50 µm. (B) Percentage of CD133+ and CD44+ cells was evaluated using flow cytometry. (C) Protein expression level of Nanog, Oct-4, SOX2 and Notch1 was observed using western blot analysis. *P<0.05 vs. control. n=3. OV, overexpression; NC, negative control; sh, short hairpin; KLK6, kallikrein 6.
